Quantification of allelic differential expression using a simple Fluorescence primer PCR-RFLP-based method
نویسندگان
چکیده
منابع مشابه
A simple, accurate and universal method for quantification of PCR.
BACKGROUND Research into gene expression enables scientists to decipher the complex regulatory networks that control fundamental biological processes. Quantitative real-time PCR (qPCR) is a powerful and ubiquitous method for interrogation of gene expression. Accurate quantification is essential for correct interpretation of qPCR data. However, conventional relative and absolute quantification m...
متن کاملMolecular identification of anaplasmosis in goats using a new PCR-RFLP method
In this study blood samples were collected from 193 goats in north and northeastern Iran with the aim to develop a PCR-RFLP assay, as a specific and sensitive diagnostic tool enabling direct and concurrent identification of two Anaplasma species (A. ovis, A. marginale) in goats. A polymerase chain reaction (PCR) for amplification of a fragment of the major surface protein 4 (msp4) gene from A....
متن کاملA Simple Genome Walking Strategy to Isolate Unknown Genomic Regions Using Long Primer and RAPD Primer
Background: Genome walking is a DNA-cloning methodology that is used to isolate unknown genomic regions adjacent to known sequences. However, the existing genome-walking methods have their own limitations. Objectives: Our aim was to provide a simple and efficient genome-walking technology. Material and Methods: In this paper, we dev...
متن کاملDifferential identification of mycoplasma pulmonis and M. arthritidis using PCR-based RFLP.
Mycoplasma pulmonis and Mycoplasma arthritidis were differentially identified using PCR-restriction fragment length polymorphism (RFLP). A genus-specific sequence of mycoplasma was amplified by PCR and the PCR products were digested with the restriction enzyme SmaI. Each PCR product from the four isolates of M. pulmonis was digested with SmaI into two fragments; however, there was no digestion ...
متن کاملA simple rapid detection method of DNA based on ligation-mediated real-time fluorescence PCR.
Polymerase chain reaction (PCR) has been widely used for detecting long chain DNA or RNA of viruses, bacteria and cytokines, but it is difficult to detect DNA or RNA with short length sequences. In this work, we developed a simple and rapid detection method for short length DNA sequences in complicated matrices based on ligation-mediated PCR. Two probes, both designed as 52 bases and respective...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Scientific Reports
سال: 2019
ISSN: 2045-2322
DOI: 10.1038/s41598-019-42815-5